Quantitative PCR: a quality control assay for estimation of viable virus content in live attenuated goat pox vaccine.

Evaluation of Humoral and Cell-mediated Immunity of Two Capripoxvirus Vaccine Strains against Lumpy Skin Disease Virus

Background and Aims: Prevention of Lumpy skin disease (LSD) in cattle is heavily dependent on vaccination. Since the genetic structure of LSD virus has the close relatedness with other Capri-pox virus (CaPV). Therefore, the use of vaccine strains of CaPV derived from sheep and goat would be useful to protect cattle against LSD. Materials and Methods: The susceptible calves of dairy farms were ...

Evaluation of Cell-mediated Immune Response in PBMCs of Calves Vaccinated by Capri Pox Vaccines Using ELISA and Real-time RT-PCR

Background: The analysis of antigen-specific cytokine expression has been considered to evaluate the immune responses and vaccines efficacy in recent years. The aim of this study was to compare the cell-mediated immune response characteristics of two Capri pox virus (CaPV) vaccines against lumpy skin disease in cattle. Materials and Methods: Two Capri pox virus vaccines were administered to da...

Development of an Attenuated Live Virus Vaccine against Sheep Pox

Production of Goat Pox Virus Vaccine from a Live Attenuated Goat Pox Virus Strain

Kid kidney cells form less than 2 months of age were grown, 80% confluent monolayers were obtained after 10-12 days. Live attenuated goat pox virus vaccine (Gorgan 55, Iranian) was used to infect the confluent monolayers. Eighty percent cytopethic effect (CPE) was obtained on day 5 of virus inoculation. TCID50 calculated for the pool virus was 10 / ml. Vaccine was found free from any bacterial ...

A new approach to develop a vaccine against capripox infection in sheep and goats using a new strain of sheep pox virus in Iran

This report describes the preparation of a safe and immunogenic single vaccine against capripox disease in sheep and goats using two different types of cells (primary lamb kidney and vero, a bovine turbinate cell line). This used the 0240 pox strain isolated from sheep during an outbreak. The prepared vaccine was administered subcutaneously and induced complete protection against an experimenta...